Molecular study of Berberis iliensis M. Pop. and Berberis sphaerocarpa Kar. et Kir. wild populations in South-East Kazakhstan using ISSR markers

Authors

  • A. S. Pozharskiy Institute of Botany and Phytointroduction, Almaty, Kazakhstan
  • S. V. Chekalin Institute of Botany and Phytointroduction, Almaty, Kazakhstan

DOI:

https://doi.org/10.26577/2218-7979-2015-8-1-15-20

Keywords:

Berberis iliensis, Berberis sphaerocarpa, Berberis oblonga, ISSR

Abstract

B. iliensis is a rare endangered plant species with decreasing natural area. Interspecific hybrids B.iliensis × B.sphaerocarpa are even more rare phenomenon. In this work 6 populations of B.iliensis, 4 populations of B. sphaerocarpa and a single B. oblonga population are analysed. DNA from barberry leaves was extracted using SDS method. ISSR analysis was conducted with 5 primers. UPGMA tree and STRUCTURE diagram of 11 populations were constructed. Obtained results show relations between B.iliensis and B. sphaerocarpa in pure and hybrid populations and lay a first basis for further investigation of Kazakhstan barberry species.

References

1 Ahrendt L. Berberis and Mahonia, a taxonomic revision// Bot. J . Linn. Soc.. – 1961. – vol.57. – 1–410.

2 Sokolov S. J. (ed.) Trees and shrubs of USSR part VII. - Leningrad: Academia of Science of USSR press., 1954. – 872 p.

3 Dzhangaliev A. D., Salova T. N. & Turekhanova P. M. The wild fruit and nut plants of Kazakhstan//Horticultural Reviews. – 2003. – №23. – 305-
371.

4 Resolution of the Government of Republic of Kazakhstan №1034. About approval of the list of rare and endangered animal and plant species. – October 31, 2006.

5 Chekalin S. V., Muhitdinov A. S., Zaychenko O. P., Nabieva S. V., Masalova V. A., Pozharskiy A.S. Natural hybridization of Berberis iliensis M. Pop. and Berberis sphaerocarpa Kar. et Kir.// Preservation and rational use of genetic fund of wild fruit forests of Kazakhstan: Proceedings of the Interanational academic conference. – Almaty, 2013. – P. 140-145.

6 Dellaporta S.L., Wood J., Hicks J. B. A plant DNA minipreparation: version II// Plant molecular biology reporter. – 1983. – Vol.1 – №4. – 19-21.

7 Wolfe A. D., Xiang Q.-Y., Kephart S. R. Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimple sequence repeat (ISSR) bands //Molecular
Ecology . – 1998. – №7. – 1107-1125.

8 Schlüter P. M., Harris S. A. Analysis of multilocus fingerprinting data sets containing missing data//Molecular Ecology Notes . – 2006. – №6. – 569-572.

9 Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0// Molecular Biology and Evolution. – 2007. – № 24. – 1596-1599.

10 Pritchard J. K., Stephens M., Donnelly P. Inference of population structure using multilocus genotype data// Genetics. – 2000. – №155. – 945-959.

11 Jakobsson M. & Rosenberg N. A. CLUMPP : a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure// Bioinformatics. – 2007. – vol.23. –1801-1806.

12 Rosenberg N. A. Distruct: a program for the graphical display of population structure// Mol.Ecol.Notes. – 2004. – vol.4. –137–138.

13 Earl, Dent A. and vonHoldt, Bridgett M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method// Conservation Genetics Resources. – 2012. – Vol. 4 (2). – Pp. 359-361.

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Published

2015-06-30

How to Cite

Pozharskiy, A. S., and S. V. Chekalin. 2015. “Molecular Study of Berberis Iliensis M. Pop. and Berberis Sphaerocarpa Kar. Et Kir. Wild Populations in South-East Kazakhstan Using ISSR Markers”. International Journal of Biology and Chemistry 8 (1):15-20. https://doi.org/10.26577/2218-7979-2015-8-1-15-20.