Molecular study of Berberis iliensis M. Pop. and Berberis sphaerocarpa Kar. et Kir. wild populations in South-East Kazakhstan using ISSR markers

  • A. S. Pozharskiy Institute of Botany and Phytointroduction, Almaty, Kazakhstan
  • S. V. Chekalin Institute of Botany and Phytointroduction, Almaty, Kazakhstan

Abstract

B. iliensis is a rare endangered plant species with decreasing natural area. Interspecific hybrids B.iliensis × B.sphaerocarpa are even more rare phenomenon. In this work 6 populations of B.iliensis, 4 populations of B. sphaerocarpa and a single B. oblonga population are analysed. DNA from barberry leaves was extracted using SDS method. ISSR analysis was conducted with 5 primers. UPGMA tree and STRUCTURE diagram of 11 populations were constructed. Obtained results show relations between B.iliensis and B. sphaerocarpa in pure and hybrid populations and lay a first
basis for further investigation of Kazakhstan barberry species.

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Published
2015-06-30
How to Cite
POZHARSKIY, A. S.; CHEKALIN, S. V.. Molecular study of Berberis iliensis M. Pop. and Berberis sphaerocarpa Kar. et Kir. wild populations in South-East Kazakhstan using ISSR markers. International Journal of Biology and Chemistry, [S.l.], v. 8, n. 1, p. 15-20, june 2015. ISSN 2409-370X. Available at: <http://ijbch.kaznu.kz/index.php/kaznu/article/view/170>. Date accessed: 24 feb. 2019. doi: https://doi.org/10.26577/2218-7979-2015-8-1-15-20.

Keywords

Berberis iliensis; Berberis sphaerocarpa; Berberis oblonga; ISSR