Influence of heavy metals on fluorescence activity of perspective strains of microalgae and cyanobacteria

B. K. Zayadan, N. R. Akmuhanova, A. K. Sadvakasova, D. K. Kirbaeva, K. Bolatkhan, M. O. Bauyenova

Abstract


The article presents the results of a study of the effect of heavy metals on fluorescence activity of microalgae and cyanobacteria. Based on the results of the study determined that the collection of strains of microalgae and cyanobacteria 5 crops resistant to heavy metals, 4 cultures are more sensitive to the studied concentrations of heavy metals. Some strains under the influence of the zinc and copper was observed phenomenon of plasmolysis and deformation of the cells. Influence of heavy metals on cells of microalgae and cyanobacteria have identified a number of surveyed metals toxicity, which is as follows: Cu2 + > Zn2 + > Co2 + > Ni2+. From cultures of microalgae and
cyanobacteria the following types of selected for further study, as promising strains for bioremediation of contaminated aquatic ecosystems, various heavy metals: Phormidium autumnale I-5, Anabaena variabilis RI-5, Synechococcus elongatus I-4, Chlorella vulgaris sp BB-2, Chlamydomonas reinhardtii B -4.

Keywords


microalgae; cyanobacteria; heavy metals

Full Text:

PDF

References


Teo S. C. and. Wong L. S, Whole Cell-based Biosensors for Environmental Heavy Metals Detection // Annual Research & Review in Biology. -2014. – Vol. 4. – pp. 2663-2674.

Choe S.I., et al., Role of Aspergillus niger acrA in arsenic resistance and its use as the basis for an arsenic biosensor. // Applied and environmental microbiology. – 2012. Vol. 78. – pp. 3855-3863.

Claude D., et al., Whole cell algal biosensors for urban waters monitoring. // in Novatech. – 2007. – pp. 1507-1514.

Costa G., et al., Advances on using a bioluminescent microbial biosensor to detect bioavailable Hg (II) in real samples // American Journal of Bioscience and Bioengineering. – 2013. – Vol. 1. – pp. 44-48.

Wong L. S., et al., Whole cell biosensor using Anabaena torulosa with optical transduction for environmental toxicity evaluation // Journal of Sensors. – 2013. – Vol., p. ID 567272.

Andersen R. Algal Culturing Techniques. – Imprint: ACADEMIC PRESS, ISBN: 978-0-12-088426-1. 2005

Zayadan B.K.,. Matorin D.N. Biomonitoring of aquatic ecosystems based on microalgae.-M.: IZD-vo “Altex” 2015. -252 p.

Rules of the Ministry of the environment from 14.06.1994 “Surface water protection regulations of the republic of Kazakhstan”

Matorin D.N., Antal T.K., Ostrowska M., Rubin A.B., Ficek D.Chlorophyll fluorometry as a method for studying light absorption by photosynthetic pigments in marine algae // Oceanologia. – 2004. – V. 46. № 4. – P. 519–531.