@article{Belkozhayev_Niyazova_Wilson_2021, title={The features of novel miRNAs interaction with mRNA candidate genes having trinucleotide repeats in coding sequences and untranslated regions}, volume={13}, url={https://ijbch.kaznu.kz/index.php/kaznu/article/view/475}, DOI={10.26577/ijbch.2020.v13.i2.04}, abstractNote={<p><span class="fontstyle0">Trinucleotide repeat disorders are a group of predominantly inherited neurological diseases<br>caused by the expansion of repetitive sequences. miRNAs play major roles in transcriptional regulation and<br>are expressed selectively and abundantly in the central nervous sy</span><span class="fontstyle2">st</span><span class="fontstyle0">em. In the present research, MirTarget<br>program predicted the miRNA-binding sites in mRNAs of genes with trinucleotide repeats. The MirTarget<br>programme determines the following features of binding: the </span><span class="fontstyle2">st</span><span class="fontstyle0">art of the initiation of miRNA binding<br>to mRNAs; the localization of miRNA binding sites in 5’UTRs, CDSs and 3’UTRs; the free energy of<br>binding; and the schemes of nucleotide interactions between miRNAs and mRNAs. In coding sequences<br>the binding sites of ID00372.5p-miR with mRNA of </span><span class="fontstyle3">ATXN2, FMN2 </span><span class="fontstyle0">and </span><span class="fontstyle3">MN1 </span><span class="fontstyle0">genes having CAG (Q)<br>repeats show the highe</span><span class="fontstyle2">st </span><span class="fontstyle0">free binding energy</span><span class="fontstyle4">. </span><span class="fontstyle0">The mRNA of </span><span class="fontstyle3">ADRBK1, BRSK2, C11orf87 </span><span class="fontstyle0">and </span><span class="fontstyle3">FMR1<br></span><span class="fontstyle0">genes have ID01508.5p-miR binding sites in 5’UTR with CGG repeated regions. Also, the binding sites<br>of ID00296.3p-miR and ID01702.3p-miR in 5’UTR of </span><span class="fontstyle3">BLMH </span><span class="fontstyle0">gene interacted with CCG repeats. </span><span class="fontstyle3">DMPK<br></span><span class="fontstyle0">gene with CUG repeated regions have ID00522.5p-miR binding sites in 3’UTR. Based on these results, the<br>interactions of ID00372.5p-miR, ID01508.5p-miR, ID00296.3p-miR, ID01702.3p-miR and ID00522.5pmiR and their target genes </span><span class="fontstyle3">ATXN2, FMN2, MN1, ADRBK1, BRSK2, C11orf87, FMR1, BLMH </span><span class="fontstyle0">and </span><span class="fontstyle3">DMPK<br></span><span class="fontstyle0">can be used for developing methods for diagnosing and therapeutic targets for neurological disorders.</span></p>}, number={2}, journal={International Journal of Biology and Chemistry}, author={Belkozhayev, A.M. and Niyazova, R.Ye. and Wilson, C.M.}, year={2021}, month={Jan.}, pages={30–37} }