Physico-chemical properties of physiologically active polysaccharides from wheat tissue culture

  • S. K. Kazybekova The Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
  • N. K. Bishimbaeyva The Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
  • A. S. Murtazina The Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
  • S. M. Tazhibaeyva Al-Farabi Kazakh National University, Almaty Kazakhstan
  • R. Miller Max Planck Institute of colloids and interfaces, Germany, Potsdam


Polysaccharides (PS) from wheat cell culture were isolated by liquid-liquid extraction. The molecular mass distribution was determined by gel-permeation chromatography (GPC) using dual detectors for the simultaneous detection. It was supposed that PS sample from wheat cell culture has molecular weight of 1632 Da. The physic-chemical properties of PS such as solubility in different solvents, surface activity, ξ-potential, the pH value, polydispersity (PDI) were determined. The PS sample was soluble in water and insoluble in ethanol, acetone and chloroform. ξ-potential of PS was evaluated in order to determine its charge at different pH value from 3 to 9. As a result, the ξ values for the PS solution were negative throughout the pH range studied, varying from -2.85 mV (pH 3.0) to -21.1 (pH 9). Using tensiometry method, surface tension of the PS at the liquid/air interface was investigated. At 0.05% concentration interfacial tension decreases slowly and reaches an equilibrium value after ~ 8-8.5 hours. The pH was equal to 5.6±0.05. For a PS solution of 0.001% at pH 5.5 PDI was equal to 0.595.

Author Biography

R. Miller, Max Planck Institute of colloids and interfaces, Germany, Potsdam
Kazybekova S.K.1, .1*, Murtazina A.S.1, Tazhibayeva S.M.2, Miller R.3


1. Gorshkova T.A., Wyatt S.E., Salnikov V.V. Cell-wall polysaccharides of developing flax plants // Plant Physiol, 1996. – Vol. 110. – P. 721-729.

2. Wu A.M. Complex Carbohydrates in Microbial and Viral infections and vaccine design // The Molecular Immunology of Complex Carbohydrates,
1997. – Vol. 2. – P. 173-176.

3. Gillis R.B., Adams G.G., Wolf B., Berry M., Besong T.D., Corfield A. et al. Molecular weight distribution analysis by ultracentrifugation: adaptation of a new approach for mucins // Carbohydrate Polymers,
2013. – Vol. 93. – P. 178-183.

4. Harding S.E., Abdelhameed A.S., Morris G.A. Molecular weight distribution, evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation // Macromolecular Bioscience, 2010. – Vol.10. – P. 714-720.

5. Jingjing Yang, Fan Yang, Huimin Yang, Guiyun Wang. Water-soluble polysaccharide isolated with alkali from the stem of Physalis alkekengi L.: Structural characterization and immunologic enhancement in DNA vaccine // Carbohydrate Polymers, 2015. – Vol.121. – P.248-253

6. Kawahara K., Ohta K., Miyamoto H., Nakamura S. Preparation and solution properties of pullulan fractions as standard samples for water-soluble polymers // Carbohydrate Polymers, 1984. – Vol.4. – P.335-356.

7. Freitas R.A., Martin S., Santos G.L., Valenga F., Buckeridge M.S., Reicher F. Physico-chemical properties of seed xyloglucans from different sources // Carbohydrate Polymers, 2005. – Vol.60. – P. 507-514.

8. Bi F., Mahmood S.J., Arman M., Taj N., Iqbal S. Physicochemical characterization and ionic studies of sodium alginate from Sargassum terrarium (brown algae) // Physics and Chemistry of Liquids, 2007, Vol. 45. – P.453-461.

9. Mikshina P.V., Idiyatullin B.Z., Petrova A.A., Shashkov A.S., Zuev Y.F., Gorshkova T.A. Physicochemical properties of complex rhamnogalacturonan I from gelatinous cell walls of flax fibers // Carbohydrate Polymers, 2015. – Vol.117. – P.853-861.

10. Carneiro-da-Cunha M.G., Cerqueira M.A., Bartolomeu W.S. Souza, Teixeira J.A., António A. Vicente Influence of concentration, ionic strength and pH on zeta potential and mean hydrodynamic diameter
of edible polysaccharide solutions envisaged for multinanolayered films production // Carbohydrate Polymers, 2011. – Vol. 85. – P.522-528.

11. Hernández-Marín N.Y., Lobato-Calleros C., Vernon-Carter E.J. Stability and rheology of waterin-oil-in-water multiple emulsions made with protein-polysaccharide soluble complexes // Journal of Food Engineering, 2013. – Vol.119. – P.181-187.

12. Murray B.S. Rheological properties of protein films //Current Opinion in Colloid and Interface Science, 2011. – Vol.16. – P.27-35.

13. Sluiter A., Hames B., Ruiz R., Scarlata C., Sluiter J., Templeton D. Determination of structural carbohydrates and lignin in biomass // Analytical Journal, 2005. – Vol.17. – P.123-125.

14. Romero A., Beaumal V., David-Briand E., Cordobes F., Guerrero A., Anton M., Interfacial and oil/water emulsions characterization of potato
protein isolates // Journal of Agricultural and Food Chemistry, 2011. – Vol. 59. – No. 17. – P. 9466-9474.
How to Cite
KAZYBEKOVA, S. K. et al. Physico-chemical properties of physiologically active polysaccharides from wheat tissue culture. International Journal of Biology and Chemistry, [S.l.], v. 8, n. 2, p. 18-22, dec. 2015. ISSN 2409-370X. Available at: <>. Date accessed: 19 apr. 2019. doi:


polysaccharide; surface activity; average molecular weight