Biotransformation of anabolic drug Dianabol with Rizhopus oryzae

Authors

  • M. Siddiqui H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan
  • Atia-tul-Wahab Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan
  • B.M. Kudaibergenova Al-Farabi Kazakh National University, Almaty, Kazakhstan
  • Zh.A. Abilov Al-Farabi Kazakh National University, Almaty, Kazakhstan
  • M.I. Choudhary H.E.J. Research Institute of Chemistry; Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan; Al-Farabi Kazakh National University, Almaty, Kazakhstan

DOI:

https://doi.org/10.26577/IJBCh2024v17i1-a12
        71 66

Abstract

Microbial biotransformation technique is an excellent approach for the synthesis of stereo-, enantio-, chemo-, and regio-selective/specific analogues of existing steroidal and non-steroidal drugs by using bacteria, fungi, algae, actinomycetes, yeast, and plants and animals cell cultures. This technique is effectively used to synthesize compounds whose structures resemble to the substrates (parent drugs) without using protecting/deprotecting steps.  In the current study, an anabolic-androgenic steroid (AAS) based drug, methandienone (dianabol) (1) was incubated with the filamentous fungi Rhizopus oryzae ATCC 11145 for twelve days under ambient reaction conditions (at room temperature and neutral pH) by using aqueous media.  Therefore, the present study has successfully helped to produce the structural analogues of inert steroidal anabolic drug dianabol (1) without using expensive and toxic chemicals. This yielded five known structural analogues of dianabol (1), i.e., 17β,11β-dihydroxy,17α-methyl-androsta-1,4-diene-3-one (2), 17β-hydroxy,17α-methyl-androsta-1,4-diene-3,11-dione (3), 17β,6β-dihydroxy,17α-methyl-androsta-1,4-diene-3-one (4), 17β,6β-dihydroxy,17α-methyl-androsta-4-ene-3-one (5), and 17β-hydroxy,17α-methyl-androsta-4,6-diene-3-one (6). Structures of transformed products 2-6 were determined through 1H-NMR, and FAB-MS spectroscopic techniques.

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How to Cite

M. Siddiqui, Atia-tul-Wahab, B.M. Kudaibergenova, Zh.A. Abilov, and M.I. Choudhary. 2024. “Biotransformation of Anabolic Drug Dianabol With Rizhopus Oryzae”. International Journal of Biology and Chemistry 17 (1):108-11. https://doi.org/10.26577/IJBCh2024v17i1-a12.