Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L.

D. N. Satybaldiyeva, V. K. Mursaliyeva, R. Mammadov, B. K. Zayadan

Abstract


The aim of this study is to investigate the phenolic profiles and evaluate the brine shrimp cytotoxic activity of the ethanolic extract from the aerial part of C. alatavicus, an endemic species of Kazakhstan flora. Nine phenolic compounds were identified and quantified in the extract by high-performance liquid chromatography method. Preliminary cytotoxicity of the extract was determined by the brine shrimp (Artemiasalina) assay. The results reveal that the ethanolic extract from the aerial part of Crocus alatavicus exhibit a high cytotoxicity with LC50 15.71 μg/mL.

Keywords


Crocusalatavicus; phenolic compound; cytotoxicity; brine shrimp; extract

Full Text:

PDF

References


Bhattacharya A., Sood P., Citovsky V. The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Mol. Plant. Pathol. 2010; 11(5): 705-719.

Saxena R.B. Botany, Taxonomy and cytology of Crocus orientales-series. Asian J Sci Tech 2015; 6 (5): 1406-1410.

Duke J.A., Ayensu E.E. Medicinal plants of China. Algona: Reference Publications; 1985.

Egamberdieva D., Mamadalieva N., Khodjimatov O., Tiezzi A. Medicinal plants from Chatkal biosphere reserve used for folk medicine in Uzbekistan. Med Aromat Plant Sci Biotech 2012; 7(1): 56-64.

Golovkin B.N., Rudenskaya R.N., Trofimova I.A., Shreter A.I. Biologicheski aktivnye veshestva rastitelnogo proiskhozhdeniya. Moscow: Nauka; 2001.

Satybaldiyeva D, Mursaliyeva V, Rakhimbayev I, Mammadov R, Zayadan B. Preliminary phytochemical

analysis and antioxidant, antibacterial activities of Crocus alatavicus from Kazakhstan. Not Bot Horti Agrobot Cluj Napoca 2015; 43 (2): 343-348.

Ivaschenko A.A. Tulips and other bulbs plants of Kazakhstan. Almaty: Printing house “Two Capitals”;

Norbak R., Brandt K., Nielsen J.K., Orgaard M., Jacobsen N. Flower pigment composition of Crocus species and cultivars used for a chemotaxonomic investigation. Biochem Syst Ecol 2002; 30: 763-791.

Caponio F., Alloggio V., Gomes T. Phenolic compounds of virgin olive oil: influence of paste preparation techniques. Food Chem 1999; 64 (2): 203-209.

Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen L.B., Nichols D.E., McLaughlin J.L. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982; 45: 31-34.

McLaughlin J.L., Chang C.J., Smith D.L. Bench-top bioassays for the discovery of bioactive natural products: An Update. In: Studies in Natural Products Chemistry, Rahman A.U. Oxford: Elsevier; 1991, pp. 383-409.

Acar G., Dogan N.M., Duru M.E., Kivrak I. Phenolic profiles, antimicrobial and antioxidant activity of various extracts of Crocus species in Anatolia. Afr J Microbiol Res 2010; 4(11): 1154-1161.

Gismondi A., Serio M., Canuti L., Canini A. Biochemical, antioxidant and antineoplastic properties of Italian saffron (Crocus sativusL.). Am J Plant Sci 2012; 3: 1573-1580

Karimi E., Oskoueian E., Hendra R., Hawa Z.E. Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules 2010; 15: 6244-6256.

Taha A., Alsayed H. Brine shrimp bioassay of ethanol extracts of Sesuvium verrucosum, Salsolaba ryosma and Zygophyllum quatarense medicinal plants from Bahrain. Phytother Res 2000; 14: 48–50.

Behravan J., Hosseinzadeh H., Rastgoo A., Obeid M., Hessani M. Evaluation of the cytotoxic activity of crocin and safranal using potato disc and brine shrimp assays. Physiol Pharmacol 2010; 13 (4): 397-403.

Hosseinzadeh H., Nassiri-Asl M. Avicenna’s (Ibn Sina) the canon of medicine and saffron (Crocus sativus): A review. Phytother Res 2013; 27: 475-483.