Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L.

  • D. N. Satybaldiyeva Al Farabi Kazakh National University, Almaty
  • V. K. Mursaliyeva Institute of Plant Biology and Biotechnology, Almaty
  • R. Mammadov Pamukkale University
  • B. K. Zayadan Al Farabi Kazakh National University, Almaty


The aim of this study is to investigate the phenolic profiles and evaluate the brine shrimp cytotoxic activity of the ethanolic extract from the aerial part of C. alatavicus, an endemic species of Kazakhstan flora. Nine phenolic compounds were identified and quantified in the extract by high-performance liquid chromatography method. Preliminary cytotoxicity of the extract was determined by the brine shrimp (Artemiasalina) assay. The results reveal that the ethanolic extract from the aerial part of Crocus alatavicus exhibit a high cytotoxicity with LC50 15.71 μg/mL.


1. Bhattacharya A., Sood P., Citovsky V. The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Mol. Plant. Pathol. 2010; 11(5): 705-719.

2. Saxena R.B. Botany, Taxonomy and cytology of Crocus orientales-series. Asian J Sci Tech 2015; 6 (5): 1406-1410.

3. Duke J.A., Ayensu E.E. Medicinal plants of China. Algona: Reference Publications; 1985.

4. Egamberdieva D., Mamadalieva N., Khodjimatov O., Tiezzi A. Medicinal plants from Chatkal biosphere reserve used for folk medicine in Uzbekistan. Med Aromat Plant Sci Biotech 2012; 7(1): 56-64.

5. Golovkin B.N., Rudenskaya R.N., Trofimova I.A., Shreter A.I. Biologicheski aktivnye veshestva rastitelnogo proiskhozhdeniya. Moscow: Nauka; 2001.

6. Satybaldiyeva D, Mursaliyeva V, Rakhimbayev I, Mammadov R, Zayadan B. Preliminary phytochemical
analysis and antioxidant, antibacterial activities of Crocus alatavicus from Kazakhstan. Not Bot Horti Agrobot Cluj Napoca 2015; 43 (2): 343-348.

7. Ivaschenko A.A. Tulips and other bulbs plants of Kazakhstan. Almaty: Printing house “Two Capitals”;

8. Norbak R., Brandt K., Nielsen J.K., Orgaard M., Jacobsen N. Flower pigment composition of Crocus species and cultivars used for a chemotaxonomic investigation. Biochem Syst Ecol 2002; 30: 763-791.

9. Caponio F., Alloggio V., Gomes T. Phenolic compounds of virgin olive oil: influence of paste preparation techniques. Food Chem 1999; 64 (2): 203-209.

10. Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen L.B., Nichols D.E., McLaughlin J.L. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982; 45: 31-34.

11. McLaughlin J.L., Chang C.J., Smith D.L. Bench-top bioassays for the discovery of bioactive natural products: An Update. In: Studies in Natural Products Chemistry, Rahman A.U. Oxford: Elsevier; 1991, pp. 383-409.

12. Acar G., Dogan N.M., Duru M.E., Kivrak I. Phenolic profiles, antimicrobial and antioxidant activity of various extracts of Crocus species in Anatolia. Afr J Microbiol Res 2010; 4(11): 1154-1161.

13. Gismondi A., Serio M., Canuti L., Canini A. Biochemical, antioxidant and antineoplastic properties of Italian saffron (Crocus sativusL.). Am J Plant Sci 2012; 3: 1573-1580

14. Karimi E., Oskoueian E., Hendra R., Hawa Z.E. Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules 2010; 15: 6244-6256.

15. Taha A., Alsayed H. Brine shrimp bioassay of ethanol extracts of Sesuvium verrucosum, Salsolaba ryosma and Zygophyllum quatarense medicinal plants from Bahrain. Phytother Res 2000; 14: 48–50.

16. Behravan J., Hosseinzadeh H., Rastgoo A., Obeid M., Hessani M. Evaluation of the cytotoxic activity of crocin and safranal using potato disc and brine shrimp assays. Physiol Pharmacol 2010; 13 (4): 397-403.

17. Hosseinzadeh H., Nassiri-Asl M. Avicenna’s (Ibn Sina) the canon of medicine and saffron (Crocus sativus): A review. Phytother Res 2013; 27: 475-483.
How to Cite
SATYBALDIYEVA, D. N. et al. Phenolic profiles and brine shrimp cytotoxicity of the ethanolic extract from the aerial part of Crocus alatavicus L.. International Journal of Biology and Chemistry, [S.l.], v. 9, n. 1, p. 38-41, jan. 2017. ISSN 2409-370X. Available at: <>. Date accessed: 16 aug. 2017.


Crocusalatavicus; phenolic compound; cytotoxicity; brine shrimp; extract