Identification of flavonoids in plant samples by gas chromatography-mass spectrometry with pre-derivatization

  • M. Alimzhanova Al-Farabi Kazakh National University
  • D. Makhayeva Al-Farabi Kazakh National University

Abstract

Plants of Artemisia L. genus are important source of flavonoids – a group of biologically active substances, which are widely used in the perfume, alcoholic beverage, pharmaceutical industry and medicine. Flavonoids have antiseptic, anti-tumor, anti-inflammatory, antibacterial, antituberculosis actions. Therefore, testing and introduction of many types of Artemisia to the culture, and their selection in Kazakhstan is up-and-coming. Qualitative composition of wormwood Artemisia scopariaWaldst. et Kit flavonoids collected in Almaty and Ile-Balkhash region in 2013 year was studied by gas chromatography-mass spectrometry method. Samples were prepared by extraction three times with 40% ethanol solution. During the identification of flavonoids by gas chromatography-mass spectrometry pre-derivatization of the compounds was carried out by N,O-Bis(trimethylsilyl) trifluoroacetamide, which is the most reactive derivatizing agent to increase the volatility and thermal stabilityofstudied substances. Kaempferol and quercetin were found as a result of analysis.

Author Biographies

M. Alimzhanova, Al-Farabi Kazakh National University
Center of physico-chemical methods of research and analysis, Almaty
D. Makhayeva, Al-Farabi Kazakh National University
Center of physico-chemical methods of research and analysis, Almaty

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How to Cite
ALIMZHANOVA, M.; MAKHAYEVA, D.. Identification of flavonoids in plant samples by gas chromatography-mass spectrometry with pre-derivatization. International Journal of Biology and Chemistry, [S.l.], v. 9, n. 1, p. 46-51, jan. 2017. ISSN 2409-370X. Available at: <http://ijbch.kaznu.kz/index.php/kaznu/article/view/211>. Date accessed: 27 june 2017.
Section
Chemistry

Keywords

analysis; Artemisia; derivatization; flavonoids; gas chromatography; kaempferol; mass spectrometry; quercetin