Identification of flavonoids in plant samples by gas chromatography-mass spectrometry with pre-derivatization

M. Alimzhanova, D. Makhayeva


Plants of Artemisia L. genus are important source of flavonoids – a group of biologically active substances, which are widely used in the perfume, alcoholic beverage, pharmaceutical industry and medicine. Flavonoids have antiseptic, anti-tumor, anti-inflammatory, antibacterial, antituberculosis actions. Therefore, testing and introduction of many types of Artemisia to the culture, and their selection in Kazakhstan is up-and-coming. Qualitative composition of wormwood Artemisia scopariaWaldst. et Kit flavonoids collected in Almaty and Ile-Balkhash region in 2013 year was studied by gas chromatography-mass spectrometry method. Samples were prepared by extraction three times with 40% ethanol solution. During the identification of flavonoids by gas chromatography-mass spectrometry pre-derivatization of the compounds was carried out by N,O-Bis(trimethylsilyl) trifluoroacetamide, which is the most reactive derivatizing agent to increase the volatility and thermal stabilityofstudied substances. Kaempferol and quercetin were found as a result of analysis.


analysis; Artemisia; derivatization; flavonoids; gas chromatography; kaempferol; mass spectrometry; quercetin

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Zhang Q, Ye M.(2009) J. Chromatogr. A.1216:1954-1969.

LinderborgK., LaaksonenA., KallioH., YangB. (2011) Food Res. Int. 44:2027-2033.

ZhengJ., YangB., TuomasjukkaS., OuSh., KallioH.(2009) J. Agric. Food Chem57:2977-2987.

Creaser C., Koupai-Abyazani M., Stephenson G.(1989) J. Chromatogrol. 478:415-421.

Fenner G. (1996) J. Agric. Food Chem. 44:3727-3729.

ZhangK., Zuo Y.(2004) J. Agric. Food Chem.52:222-227.

AnliErtan, VuralN., Kizilet E.(2008) JI Brewing. 114:239-245

Garcia-VillalbaR., PacchiarottaT., Carrasco-PancorboA., Segura-CarreteroA., Fernandez-GutierrezA., DeciderA., MayborodaO.(2011) J.Chromatogr.1218:959-971.

Loots D., van der WesthuizenF., BotesL.(2007) J. Agric. Food Chem. 55:6891-6896.

Soleas G., Diamandis E., Karumanchiri A., Goldberg D.(1997) Anal. Chem. 69:4405-4409.

Horvat R., Senter S.(1980) J. Agric. Foal Chem. 28:1292-1295.

Razborsek M., Voncina D., DolecekV., Voncina E.(2007) ActaChim. Slov. 54:60-67.

Kashutin M., Ioffe S., Tartakovsky V.(1975) Usp9:1620-1648.