Production of wheat doubled haploids by in vitro anther culture

  • S. K. Turasheva Al-Farabi Kazakh National University, Almaty


The in vitro culture response of anthers, originating from high yielding and resistance to lodging F1 soft wheat hybrid
plants selected under drought conditions, was investigated. The spikes were collected when the microspores were at
the mid-uninucleate stage and after 3 days at 40 C for cold pre-treatment, the anthers were cultured in N6 and Gamborg-
Eveleg B5 (B5) solid medium. Progeny from 17 different F1 families were used and 9 of them responded to in
vitro anther culture and 7 of them produced fertile green plants. Doubled haploid green plants were fertile and produced seeds. Induction of aploid development depends on genotype, stage of male gametophyte development, composition of nutrient media and conditions of cultivation. The effect of the composition of media and different genotypes on wheat anther culture was investigated. From the genotypes produced regenerants, the high yielding genotypes from local breeding collection of wheat produced more albino plants (35.2%) compared to high yielding genotypes from CYMMIT collection (24.92%) ones. Independent of culture medium composition a ratio of doubled haploid/haploid was 4.57:1 and a ratio of haploid/aneuploid was 18.5:1, in particular 81.28 % of green plants were doubled haploid, 17.76% were haploid and the rest (0.96%) were aneuploid


1 Pickering RA, Devaux P. Haploid production: approaches and use in plant breeding. In: Genetics, Molecular Biology and Biotechnology, Barley. Ed. by PR Shewry, – Wallingford; CAB Internat., 1992. – P. 511-539.

2 Kao KN. Future prospects for crop improvement through anther and microspore culture. In: In vitro haploid production in higher plants (Jain S.M., Sopory S.K., Veilleux R.E., eds), 1996. – P. 367-373

3 Islam SM. The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.) // Aust J Crop Sci. – 2010. – V.4 (9). – P.660-665

4 Lazaridou TB, Lithourgidis AS, Kotzamanidis ST, Roupakias DG. Anther culture response of barley genotypes to cold pretreatments and culture media //Rus J Plant Physiol. – 2005. – N 52. – P. 696-699.

5 Lakin N.L. Introduction in biometry. – M.: Nauka, 1978. – 84 p.

6 Wietholter P., Fernandes MI, Brammer SP. Genotyping differences in proembyoid development and green plantlets regeneration through androgenesis in barley varieties // Ciencia Rural. – 2008. – V. 38. – P. 240-242

7 Cistue L, Valles MP, Echavarii B, Sanz JM, Castillo AM. Production of barley doubled haploids by anther and microspore culture. In: Mujib A.,
Cho MJ, Predieri S, Banerjee S In vitro application in crop improvement. – Science Publishers Inc, Plymouth, 2004. – P. 1-17

8 Turasheva SK, Zhumabaeva BA, Zhambakin KZh, Rakhimbaev IR. The influence of auxin 2.4-D on growth of wheat suspension culture //Abstract of the second Central Asian Cereals Conference. CYMMIT. – Almaty, 2006. – P. 452-455.
How to Cite
TURASHEVA, S. K.. Production of wheat doubled haploids by in vitro anther culture. International Journal of Biology and Chemistry, [S.l.], v. 8, n. 1, p. 21-25, june 2015. ISSN 2409-370X. Available at: <>. Date accessed: 19 sep. 2020. doi:


Anther culture; wheat; morphogenesis; doubled haploids; drought resistance