Сhemical composition of polysaccharides from wheat cell culture
DOI:
https://doi.org/10.26577/2218-7979-2015-8-2-13-17Keywords:
plant polysaccharides, chemical composition, wheat cell cultureAbstract
Polysaccharides were extracted from wheat suspension culture and cultivated cells. Gaz-liquid chromatography of wheat suspension culture allowed identifying the composition of polysaccharides that were found to contain arabinose, xylose, galactose, glucose, and a little amount of mannose and rhamnose. In dried cultivated cells the polysaccharide structure consisted mainly from arabinose, xylose, and galactose. It was found that the percentage of the amount of monosaccharides was positively correlated with the concentration of 2,4-D at the suspension media.References
1. Waggner H., Stuppner H., Schafer W., Zenk M. Immunologically active polysaccharides of Echinacea cell culture // Phytochemistry, 1988. –
Vol. 27. – P. 119-126.
2. Roesler J., Emmensuffer A., Steinmiller C., Luetting B., Wagner H., Lohman-Matthes M.L. Application of purified polysaccharides from cell cultures of the plant Echinacea purpurea to test subjects mediated activation of the phagocyte system // Int.J.Immunopharmac, 1991. – Vol. 13. – P. 931-941.
3. Ovodov U.S. Polysaccharides of flowering plants: structure and physiological activity // Bioorganic chemistry, 1998. – Vol. 24. – P. 483-501.
4. Goubet F., Morgan C., Synthesis of Cell Wall Galactans from Flex (Linumusitatassimum L.) Suspension-cultures Cells // Plant and Cell Physiology, 1994. – Vol. 35. – No. 5. – P. 729-727.
5. Gunter E.A., Popeyko O.V., Ovodov Yu.S., Modification of polysaccharides from callus culture of Silene vulgaris M. using carbohydrases in vitro // Biochemistry, 2007. – Vol. 72. – No. 9. – P. 1008-1015.
6. Olennikov D.N., Kashenko N.I. Polysaccharides. Modern state of research: experimentalscientific study // Chemistry of natural raw materials. 2014. – No. 1. – P. 5-26.
7. Gunter Е.А. Pectin compounds of plant cellular cultures. Thesis. Syktyvkar, 2012.
8. Bishimbayeva N.К., Denebayeva М.G., Amirova А.К., Rakhimova Е.V. Features of histological structure of friable embryogenic callus of barley // Proceedings of the National Academy of Sciences of the Republic of Kazakhstan: Series biological and medical, 2001. – No. 1-2. – P. 7-14.
9. Bishimbayeva N.K., Amirov A.K., Murtazina A.S., McDougall G.J., Rakhimbayev I.R. Biological activity of extracellular polysaccharides
in suspension culture cells of wheat // Physiological, biochemical and genetic and breeding plant research in Kazakhstan / Collection of articles, 2010 – P. 103-111.
10. Bishimbaeyva N.K. Cytophysiologic basics of long-term regeneration biotechnology in tissue culture of cereals / Synopsis for the doctor of biological sciences defense, Almaty, 2007, 38 p.
11. Murashige T., Scoog F.A. revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol.Plant, 1962. Vol. 13. – P. 473-497.
12. Gunter E.A. Non-traditional plant’s cell cultures as polysaccharides producers // Chemistry and computer modeling. Butler messages, 2001. – Vol. 5. – P. 11-12.
13. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent // J. Biol. Chem., 1951. – Vol. 193. – P. 265-275.
14. Dubious M., Gilles K. A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances // Anal. Chem., 1956. – Vol. 28. – P. 350-356.
15. Arjon J. van Hengel, Zewdie Tadesse, Peter Immerzeel, Henk Schols, Ab van Kammen, Sacco C. de Vries. N-acetylglucoseamine and Glucosamine-Containing Arabinogalactan proteins Control Somatic Embryogenesis // Plant Physiol., 2001. – Vol. 125. – P. 1880-1890.
16. McDougall G. J., Fry S.C. Structure-activity relationships for xyloglucan oligosaccharides with antiauxin activity // Plant Physiol, 1989. – Vol. 89. –P. 883
Vol. 27. – P. 119-126.
2. Roesler J., Emmensuffer A., Steinmiller C., Luetting B., Wagner H., Lohman-Matthes M.L. Application of purified polysaccharides from cell cultures of the plant Echinacea purpurea to test subjects mediated activation of the phagocyte system // Int.J.Immunopharmac, 1991. – Vol. 13. – P. 931-941.
3. Ovodov U.S. Polysaccharides of flowering plants: structure and physiological activity // Bioorganic chemistry, 1998. – Vol. 24. – P. 483-501.
4. Goubet F., Morgan C., Synthesis of Cell Wall Galactans from Flex (Linumusitatassimum L.) Suspension-cultures Cells // Plant and Cell Physiology, 1994. – Vol. 35. – No. 5. – P. 729-727.
5. Gunter E.A., Popeyko O.V., Ovodov Yu.S., Modification of polysaccharides from callus culture of Silene vulgaris M. using carbohydrases in vitro // Biochemistry, 2007. – Vol. 72. – No. 9. – P. 1008-1015.
6. Olennikov D.N., Kashenko N.I. Polysaccharides. Modern state of research: experimentalscientific study // Chemistry of natural raw materials. 2014. – No. 1. – P. 5-26.
7. Gunter Е.А. Pectin compounds of plant cellular cultures. Thesis. Syktyvkar, 2012.
8. Bishimbayeva N.К., Denebayeva М.G., Amirova А.К., Rakhimova Е.V. Features of histological structure of friable embryogenic callus of barley // Proceedings of the National Academy of Sciences of the Republic of Kazakhstan: Series biological and medical, 2001. – No. 1-2. – P. 7-14.
9. Bishimbayeva N.K., Amirov A.K., Murtazina A.S., McDougall G.J., Rakhimbayev I.R. Biological activity of extracellular polysaccharides
in suspension culture cells of wheat // Physiological, biochemical and genetic and breeding plant research in Kazakhstan / Collection of articles, 2010 – P. 103-111.
10. Bishimbaeyva N.K. Cytophysiologic basics of long-term regeneration biotechnology in tissue culture of cereals / Synopsis for the doctor of biological sciences defense, Almaty, 2007, 38 p.
11. Murashige T., Scoog F.A. revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol.Plant, 1962. Vol. 13. – P. 473-497.
12. Gunter E.A. Non-traditional plant’s cell cultures as polysaccharides producers // Chemistry and computer modeling. Butler messages, 2001. – Vol. 5. – P. 11-12.
13. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent // J. Biol. Chem., 1951. – Vol. 193. – P. 265-275.
14. Dubious M., Gilles K. A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances // Anal. Chem., 1956. – Vol. 28. – P. 350-356.
15. Arjon J. van Hengel, Zewdie Tadesse, Peter Immerzeel, Henk Schols, Ab van Kammen, Sacco C. de Vries. N-acetylglucoseamine and Glucosamine-Containing Arabinogalactan proteins Control Somatic Embryogenesis // Plant Physiol., 2001. – Vol. 125. – P. 1880-1890.
16. McDougall G. J., Fry S.C. Structure-activity relationships for xyloglucan oligosaccharides with antiauxin activity // Plant Physiol, 1989. – Vol. 89. –P. 883
Downloads
Published
2015-12-27
How to Cite
Bishimbayeva, N. K., I. A. Sartbayeva, A. S. Murtazina, and E. A. Gunter. 2015. “Сhemical Composition of Polysaccharides from Wheat Cell Culture”. International Journal of Biology and Chemistry 8 (2):13-17. https://doi.org/10.26577/2218-7979-2015-8-2-13-17.
Issue
Section
Biology
License
ааа
