Utilization of peanut shells as substrate for cellulase production in submerged fermentation through Box-Behnken Design

  • M. Irfan University of Sargodha
  • S. sadia University of Sargodha
  • J. Bakhtawar University of Sargodha
  • H. A. Shakir University of the Punjab new campus
  • M. Khan University of the Punjab new campus
  • S. Ali Government College University

Abstract

Increasing environmental pollution and global warming encourage bioengineers andbiotechnologists to find renewable and environmental friendly sources of energy. Conversion of biomassespecially agro-industrial biomass consisting of lignocellulosic material into different products andenergy is the modern and promising scheme for this purpose due to their abundance in nature, lower costand easy handling. These properties made them potential producers of biofuels in near future. In currentresearch, Bacillus paralichniformis potential for cellulase production was analyzed using submergedfermentation, for which peanut shell waste was used as a substrate. Nutritional factors were optimized forbest enzyme production using Box-Behnken Design (BBD) of response surface methodology (RSM).Results illustrated the maximum enzyme production of 12.838 IU for CMCase and 40.956 IU for FPaseafter 24 h of fermentation period. These results were obtained at 3 g/mL susbtrate concentration, 0.45g/mL yeast extract and 0.01 g/mL MgSO, for CMCase and 3g/mL substrate, 0.45 g/mL yeast extract and0.3 g/mL MgSO, for FPase. These results proposed the possibility of utilization of this strain forproduction of cellulase to endorse its use in industry.
Published
2020-01-15
How to Cite
IRFAN, M. et al. Utilization of peanut shells as substrate for cellulase production in submerged fermentation through Box-Behnken Design. International Journal of Biology and Chemistry, [S.l.], v. 12, n. 2, p. 28-39, jan. 2020. ISSN 2409-370X. Available at: <https://ijbch.kaznu.kz/index.php/kaznu/article/view/391>. Date accessed: 25 nov. 2020. doi: https://doi.org/10.26577/ijbch-2019-i2-4.