The features of novel miRNAs interaction with mRNA candidate genes having trinucleotide repeats in coding sequences and untranslated regions

Abstract

Trinucleotide repeat disorders are a group of predominantly inherited neurological diseasescaused by the expansion of repetitive sequences. miRNAs play major roles in transcriptional regulation andare expressed selectively and abundantly in the central nervous system. In the present research, MirTargetprogram predicted the miRNA-binding sites in mRNAs of genes with trinucleotide repeats. The MirTargetprogramme determines the following features of binding: the start of the initiation of miRNA bindingto mRNAs; the localization of miRNA binding sites in 5’UTRs, CDSs and 3’UTRs; the free energy ofbinding; and the schemes of nucleotide interactions between miRNAs and mRNAs. In coding sequencesthe binding sites of ID00372.5p-miR with mRNA of ATXN2, FMN2 and MN1 genes having CAG (Q)repeats show the highest free binding energy. The mRNA of ADRBK1, BRSK2, C11orf87 and FMR1genes have ID01508.5p-miR binding sites in 5’UTR with CGG repeated regions. Also, the binding sitesof ID00296.3p-miR and ID01702.3p-miR in 5’UTR of BLMH gene interacted with CCG repeats. DMPKgene with CUG repeated regions have ID00522.5p-miR binding sites in 3’UTR. Based on these results, theinteractions of ID00372.5p-miR, ID01508.5p-miR, ID00296.3p-miR, ID01702.3p-miR and ID00522.5pmiR and their target genes ATXN2, FMN2, MN1, ADRBK1, BRSK2, C11orf87, FMR1, BLMH and DMPKcan be used for developing methods for diagnosing and therapeutic targets for neurological disorders.
Published
2021-01-03
How to Cite
BELKOZHAYEV, A.M.; NIYAZOVA, R.Ye.; WILSON, C.M.. The features of novel miRNAs interaction with mRNA candidate genes having trinucleotide repeats in coding sequences and untranslated regions. International Journal of Biology and Chemistry, [S.l.], v. 13, n. 2, p. 30-37, jan. 2021. ISSN 2409-370X. Available at: <https://ijbch.kaznu.kz/index.php/kaznu/article/view/475>. Date accessed: 17 apr. 2021. doi: https://doi.org/10.26577/ijbch.2020.v13.i2.04.