Decellularization of bone tissue in a supercritical carbon dioxide environment

Authors

  • R.R. Tokpayev Center of physico-chemical methods of research and analysis, al-Farabi Kazakh National University, Almaty, Kazakhstan
  • E.R. Shreider Center of physico-chemical methods of research and analysis, al-Farabi Kazakh National University, Almaty, Kazakhstan
  • Z.T. Ibraimov Center of physico-chemical methods of research and analysis, al-Farabi Kazakh National University, Almaty, Kazakhstan
  • T.M. Shalakhmetova School of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan
  • M.K. Nauryzbayev Center of physico-chemical methods of research and analysis, al-Farabi Kazakh National University, Almaty, Kazakhstan

DOI:

https://doi.org/10.26577/IJBCh2023v16i2a2
        159 295

Abstract

The use of pig bone tissue as a graft makes it possible to obtain high-quality, relatively cheap and biocompatible ECM. This is since the mineral composition of pork bone is most similar to the mineral composition of human bone. Decellularization in SC-CO2 is a promising direction, since carbon dioxide easily diffuses into the depth of the cell and is a good solvent for lipids, it is non-flammable, non-toxic and chemically non-aggressive. In this paper, the ECM in SC-CO2 was obtained in three ways: 1) in dynamic mode with large SC-CO2 flows; 2) in static and dynamic modes with large SC-CO2 flows; 3) in static and dynamic modes with large SC-CO2 flows, with preliminary exposure in ethyl alcohol as a co-solvent. According to the histological examination, the removal of ICC by the first method is 55%. The use of a co-solvent before starting the decellularization process increases the percentage of ICC removal to 98%, which allows the use of ECM as a transplant.

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How to Cite

Tokpayev, R.R., E.R. Shreider, Z.T. Ibraimov, T.M. Shalakhmetova, and M.K. Nauryzbayev. 2023. “Decellularization of Bone Tissue in a Supercritical Carbon Dioxide Environment”. International Journal of Biology and Chemistry 16 (2):16-26. https://doi.org/10.26577/IJBCh2023v16i2a2.