Dairy productivity genetic potential of holstein and brown breeds Sires in Kazakhstan
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Abstract
The research focuses on the identification of desirable gene’s alleles responsible for milk quality and production. DNA was extracted from 11 blood samples of 5 Holstein (“Sayram” local ecotype) and 6 Brown breed (“Ak-Yrys” local ecotype) for identification and genotyping of kappacasein, thyroglobulin, prolactin and growth hormone genes by polymerase chain reaction restriction fragment length polymorphism (PCRRFLP) assay. The frequency for GG, GA and AA genotypes of prolactin gene was found to be 0.091, 0.182 and 0.727, respectively. The allele frequency for G and A was 0.901 and 0.081, respectively. Frequencies for BB, AB and AA genotypes of kappa-casein were 0.091, 0.182 and 0.727, respectively. The allele frequency for A and B was 0.901 and 0.081, respectively. The frequency for CC, CT and TT genotypes of thyroglobulin gene was found to be 0.636, 0.273 and 0.088, respectively. The allele frequency for C and T was 0.901 and 0.081, respectively. The frequency for VV, VL and LL genotypes of growth hormone gene were 0.545, 0.366 and 0.089, respectively. The allele frequency for V and L was 0.901 and 0.081, respectively. According to previous studies GG genotypes of prolactin gene, BB genotypes of kappacasein gene, TT genotypes of thyroglobulin and LL genotypes of growth hormone gene are an important factors in increasing milk yield and quality and the results of this study could be used in creating the next generation of valuable animals.References
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2008;31(4):893-897
2 Torehanov A.A., Musabayev B.I., Vetrinskaya A.A., Orazymbetova Z. AFLP method for identifi cation and traceability of equine DNA. Guidance. – Almaty 2011.
3 Maniatis T., Fritsch E.F., Sambrook J. Molecular cloning: A laboratory manual. Cold Spring Harbor Press, NY. 1982
4 Scheepers RC, Marle-Köster E, Visser C (2010). Genetic variation in the kappa-casein gene of South African goats. Small Rumin. Res. 93: 53-
56.
5 Azevedo ALS, Nascimento CS, Steinberg RS, Carvalho MRS, Peixoto MGCD, Teodoro RL, Verneque RS, Guimarães SEF, Machado MA (2008).
Genetic polymorphism of the kappa-casein gene in Brazilian cattle. Gen. Mol. Res. 7(3): 623-630.
6 Patel RK, Chauhan JB, Singh KM, Soni KJ (2007). Allelic Frequency of kappa-Casein and Beta-Lactoglobulin in Indian Crossbred (Bostaurus × Bos indicus) Dairy Bulls. Turk. J. Vet. Anim. Sci. 31(6): 399-402.
7 Botaro BG, Real de Lima YV, Cortinhas CS, Prada e Silva LF, Rennó FP, Veiga dos Santos M (2009). Effect of the kappa-casein gene polymorphism, breed and seasonality on physicochemical characteristics, composition and stability of bovine milk. R. Bras. Zootechnology. 38(12): 2447-2454.
8 Yudina I.G., Turkova S.O., Kostyuchenko M.V., Lebedeva L.A., Sulimova G.E. Polymorphism of prolactin gene (microsatellites, PCR-RFLP) in
cattle. Genetics. 2001. V. 37. P. 511-516.
9 Akers R.M., Bauman D.E., Capuco A.V., Goodman G.T. & Tucker H.A. Prolactin regulationof milk secretion and biochemical differentiation of mammary epithelial cells in periparturient cows. Endocrinology. 1981. V. 109. P. 23 30.
10 Cowan С. M., Dentine M. R., Ax R. L., Schuler L. A., Restriction fragment length polymorphism associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele. Animal Genetics. 1989. V. 20. P. 157-165.
2008;31(4):893-897
2 Torehanov A.A., Musabayev B.I., Vetrinskaya A.A., Orazymbetova Z. AFLP method for identifi cation and traceability of equine DNA. Guidance. – Almaty 2011.
3 Maniatis T., Fritsch E.F., Sambrook J. Molecular cloning: A laboratory manual. Cold Spring Harbor Press, NY. 1982
4 Scheepers RC, Marle-Köster E, Visser C (2010). Genetic variation in the kappa-casein gene of South African goats. Small Rumin. Res. 93: 53-
56.
5 Azevedo ALS, Nascimento CS, Steinberg RS, Carvalho MRS, Peixoto MGCD, Teodoro RL, Verneque RS, Guimarães SEF, Machado MA (2008).
Genetic polymorphism of the kappa-casein gene in Brazilian cattle. Gen. Mol. Res. 7(3): 623-630.
6 Patel RK, Chauhan JB, Singh KM, Soni KJ (2007). Allelic Frequency of kappa-Casein and Beta-Lactoglobulin in Indian Crossbred (Bostaurus × Bos indicus) Dairy Bulls. Turk. J. Vet. Anim. Sci. 31(6): 399-402.
7 Botaro BG, Real de Lima YV, Cortinhas CS, Prada e Silva LF, Rennó FP, Veiga dos Santos M (2009). Effect of the kappa-casein gene polymorphism, breed and seasonality on physicochemical characteristics, composition and stability of bovine milk. R. Bras. Zootechnology. 38(12): 2447-2454.
8 Yudina I.G., Turkova S.O., Kostyuchenko M.V., Lebedeva L.A., Sulimova G.E. Polymorphism of prolactin gene (microsatellites, PCR-RFLP) in
cattle. Genetics. 2001. V. 37. P. 511-516.
9 Akers R.M., Bauman D.E., Capuco A.V., Goodman G.T. & Tucker H.A. Prolactin regulationof milk secretion and biochemical differentiation of mammary epithelial cells in periparturient cows. Endocrinology. 1981. V. 109. P. 23 30.
10 Cowan С. M., Dentine M. R., Ax R. L., Schuler L. A., Restriction fragment length polymorphism associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele. Animal Genetics. 1989. V. 20. P. 157-165.
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Zbrodko, Y. A., D. A. Yerezhepov, B. Zh. Baltabaev, A. M. Zhomartov, and A. S. Musayeva. 2012. “Dairy Productivity Genetic Potential of Holstein and Brown Breeds Sires in Kazakhstan”. International Journal of Biology and Chemistry, no. 4 (December):46-51. https://ijbch.kaznu.kz/index.php/kaznu/article/view/66.
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