Dairy productivity genetic potential of holstein and brown breeds Sires in Kazakhstan

Abstract

The research focuses on the identification of desirable gene’s alleles responsible for milk quality and production. DNA was extracted from 11 blood samples of 5 Holstein (“Sayram” local ecotype) and 6 Brown breed (“Ak-Yrys” local ecotype) for identification and genotyping of kappacasein, thyroglobulin, prolactin and growth hormone genes by polymerase chain reaction restriction fragment length polymorphism (PCRRFLP) assay. The frequency for GG, GA and AA genotypes of prolactin gene was found to be 0.091, 0.182 and 0.727, respectively. The allele frequency for G and A was 0.901 and 0.081, respectively. Frequencies for BB, AB and AA genotypes of kappa-casein were 0.091, 0.182 and 0.727, respectively. The allele frequency for A and B was 0.901 and 0.081, respectively. The frequency for CC, CT and TT genotypes of thyroglobulin gene was found to be 0.636, 0.273 and 0.088, respectively. The allele frequency for C and T was 0.901 and 0.081, respectively. The frequency for VV, VL and LL genotypes of growth hormone gene were 0.545, 0.366 and 0.089, respectively. The allele frequency for V and L was 0.901 and 0.081, respectively. According to previous studies GG genotypes of prolactin gene, BB genotypes of kappacasein gene, TT genotypes of thyroglobulin and LL genotypes of growth hormone gene are an important factors in increasing milk yield and quality and the results of this study could be used in creating the next generation of valuable animals.