Antimicrobial, antifungal, antioxidant activity and phytochemical investigation of fatty acids by GS/MS of raspberry (Rubus idaeus L.) shoot lipophilic extract

Abstract

The aim of the study was to determine the content of fatty acids using GC-MS in the obtained extract, conduct a study of the antimicrobial, antifungal and antioxidant activities of Rubus idaeus shoot lipophilic extract. The quantification of fatty acids was accomplished through GC-MS, antioxidant activity was assessed by potentiometric method, antimicrobial and antifungal activity was determined by well method. The 18 compounds were identified by the GS-MS. The levulenic acid (64.47±0.08 mg/100 g), linoleic acid (8.50±0.08 mg/100 g) and linolenic acid (6.80±0.04 mg/100 g) dominated in the obtained lipophilic R. idaeus shoot extract. Bacillus subtilis (17.00±0.50 mm) was the most sensitive to lipophilic extract whereas P. vulgaris was the most resistant to the lipophilic extract. Moreover, Candida albicans was medium sensitive to lipophilic extract (13.50±0.50 mm). The antioxidant activity was 1.00 mmol equiv./mdry res, according to Maslov`s antioxidant level classification it has low level. The extract exhibited antimicrobial activity against all tested strains, with the most significant impact observed against B. subtilis. However, the obtained lipophilic extract showed a relatively low level of antioxidant activity. Consequently, the derivatives of fatty acids play a substantial role in the antimicrobial and antifungal effects, whereas their contribution to antioxidant activity appears to be limited.