Evaluation of Different Sweetener Concentrations in Beverage Samples in Isfahan Market Using High Performance Liquid Chromatography

Authors

  • Seyed Mohamad Reza Nazifi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0002-8562-3957
  • Narges Esfandiarpour Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0008-5627-5288
  • Azadeh Motamedi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0002-6547-287X
  • Elham Jahanmard Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0001-6742-3444
  • Narjes Rahimi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0002-4825-4582
  • Fatemeh Ansari Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0005-7013-9010
  • Fatemeh Masoudi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0005-6523-6537
  • Soheila Sepahi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0003-0548-1344

DOI:

https://doi.org/10.26577/IJBCh20251819

Abstract

Sweeteners were added in beverage formulations to provide sweetness with pleasant taste. In this paper, potent approach for quantification of acesulfame ok (ACE-ok), sodium saccharin (Na-SAC) and aspartame (ASP) contents in beverages had been applied with liquid chromatography (RP – HPLC) joint with diode array detection (DAD). Ninety-seven samples have been selected from Isfahan marketplace. Liquid chromatograph Agilent 1260 Infinity series was applied for analysis. Mobile phase was decided on as acetonitrile and phosphate buffer (pH= 3.5) with 15:85 extent ratio (V/V), and the float rate of the chromatographic device was Optimized at 1 ml min-1 using an isocratic elution system. All the beverages were saccharin-free and the mean concentrations of acesulfame and aspartame were lower than the general standards of the Iran National Standards Organization (600 mg L-1). All the products were showed the acceptable levels of sweeteners, but, periodical and exact evaluation of these sweeteners in the beverages are still suggested to provide consumer health.

Key words: Saccharine, aspartame, acesulfame, beverages, HPLC.

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Published

2025-06-30

Issue

Vol. 18 No. 1 (2025): International Journal of Biology and Chemistry

Section

Chemistry

License

Copyright (c) 2025 International Journal of Biology and Chemistry

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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How to Cite

“Evaluation of Different Sweetener Concentrations in Beverage Samples in Isfahan Market Using High Performance Liquid Chromatography”. 2025. International Journal of Biology and Chemistry 18 (1): 100-105. https://doi.org/10.26577/IJBCh20251819.