Evaluation of Different Sweetener Concentrations in Beverage Samples in Isfahan Market Using High Performance Liquid Chromatography

S.M.R.  Nazifi; N. Esfandiarpour; A. Motamedi; E. Jahanmard; N. Rahimi; F. Ansari; F. Masoudi; S. Sepahi

doi:10.26577/IJBCh20251819

Authors

Seyed Mohamad Reza Nazifi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0002-8562-3957
Narges Esfandiarpour Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0008-5627-5288
Azadeh Motamedi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0002-6547-287X
Elham Jahanmard Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0001-6742-3444
Narjes Rahimi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0002-4825-4582
Fatemeh Ansari Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0005-7013-9010
Fatemeh Masoudi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0009-0005-6523-6537
Soheila Sepahi Laboratories of Food and Drug Control, Vice Chancellery for Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran https://orcid.org/0000-0003-0548-1344

DOI:

https://doi.org/10.26577/IJBCh20251819

Abstract

Sweeteners were added in beverage formulations to provide sweetness with pleasant taste. In this paper, potent approach for quantification of acesulfame ok (ACE-ok), sodium saccharin (Na-SAC) and aspartame (ASP) contents in beverages had been applied with liquid chromatography (RP – HPLC) joint with diode array detection (DAD). Ninety-seven samples have been selected from Isfahan marketplace. Liquid chromatograph Agilent 1260 Infinity series was applied for analysis. Mobile phase was decided on as acetonitrile and phosphate buffer (pH= 3.5) with 15:85 extent ratio (V/V), and the float rate of the chromatographic device was Optimized at 1 ml min^-1 using an isocratic elution system. All the beverages were saccharin-free and the mean concentrations of acesulfame and aspartame were lower than the general standards of the Iran National Standards Organization (600 mg L^-1). All the products were showed the acceptable levels of sweeteners, but, periodical and exact evaluation of these sweeteners in the beverages are still suggested to provide consumer health.

Key words: Saccharine, aspartame, acesulfame, beverages, HPLC.

Evaluation of Different Sweetener Concentrations in Beverage Samples in Isfahan Market Using High Performance Liquid Chromatography

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