Frequency of vernalization requirement associated dominant VRN-A1 gene and earliness related Esp-A1 candidate genes in advanced wheat mutant lines and effect of allele on flowering time

  • S. Kenzhebayeva Kazakh National University named after al-Farabi
  • G. Doktyrbay Kazakh National University named after al-Farabi
  • N. Omirbekova Kazakh National University named after al-Farabi
  • F. Sarsu Plant Breeding and Genetics Section, Joint FAO/IAEA Division, IAEA- Vienna
  • D. Tashenev Kazakh National University named after al-Farabi
  • A. Aibekova Kazakh IAEA- v. Almalybak

Abstract

Seeds of the spring bread wheat variety Zhenis, registered in Kazakhstan, were irradiated with 100 and 200 Gray doses from a [60Co] γ-ray radiation source. Promising advanced M5 mutant lines were obtained and evaluated for mapping density of vernalization requirement associated dominant VRN-A1 gene and earliness related Esp-A1 candidate genes in 30 spring wheat mutant lines along with parent. Differences among generated mutant lines and parent were observed on frequency of distribution of VRN-A1 gene and Eps-A1 candidate gene alleles. Allelic variation at the VRN-A1 loci was observed in the 8 mutant lines (26.6%), mostly in 100 Gy mutant lines. A dominant (VRN) allele at its loci, which was present and indicated in these lines is sufficient to confer a spring growth habit. As for the candidate gene Esp-A1, eleven 100 Gy M5 mutant and six 200 Gy M5 lines, had Esp-A1 candidate gene allele. The two lines24(2) and 43(4) carrying dominant allele of VRN-A1 had higher thousand kernel weight. Mutant lines carrying allele of Eps-A1 candidate gene found significantly differed from the parent especially in 100 Gy lines (73.3%) in flowering time.

Author Biographies

S. Kenzhebayeva, Kazakh National University named after al-Farabi
Molecular Ecophysiology Laboratory, Department of Biotechnology
G. Doktyrbay, Kazakh National University named after al-Farabi
Molecular Ecophysiology Laboratory, Department of Biotechnology
N. Omirbekova, Kazakh National University named after al-Farabi
Molecular Ecophysiology Laboratory, Department of Biotechnology
D. Tashenev, Kazakh National University named after al-Farabi
Molecular Ecophysiology Laboratory, Department of Biotechnology

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Published
2016-06-30
How to Cite
KENZHEBAYEVA, S. et al. Frequency of vernalization requirement associated dominant VRN-A1 gene and earliness related Esp-A1 candidate genes in advanced wheat mutant lines and effect of allele on flowering time. International Journal of Biology and Chemistry, [S.l.], v. 9, n. 1, p. 24-30, june 2016. ISSN 2409-370X. Available at: <http://ijbch.kaznu.kz/index.php/kaznu/article/view/207>. Date accessed: 25 june 2018. doi: https://doi.org/10.26577/2218-7979-2016-9-1-24-30.

Keywords

spring wheat; mutation breeding; M5 mutant lines; VRN-A1 allelic variation; Eps-Am1 candidate gene allele