Biochemical assessment of the effects of crude oil and ciprofloxacin intoxication on liver of male rats

  • K. E. Mahmoud Minoufiya University
  • T. M. Shalahmetova Al-Farabi Kazakh National University
  • B. A. Umbayev Al-Farabi Kazakh National University


Biochemical techniques were used to investigate the development of hepatoxic effects caused by single exposure to crude oil (CO, 3.0 ml/kg bw) and/or ciprofloxacin (CFX, 200 mg/kg bw) in albino rats. The results showed that hepatic P-450 1A content in crude oil exposed rats was induced after 1 day to 4 fold and reached maximum 7.6 fold after 2 days compared with control and then fell to the first day degree. CFX exposed rats significantly inhibit the hepatic P-450 1A content while in crude oil and CFX co-exposed group it induced but in lesser degree than that induced by crude oil alone. Inhibition of cytochrome P-450 1A has no effect on the activity of lipid peroxidation (LPO) while its activation latter was caused by elimination of the inhibitor from the body and delayed activation of cytochrome P-450 1A. Single exposure of both crude oil and CFX lead to destructive changes in liver cells and inhibition of CYP1A by ciprofloxacin could not protect against lipid peroxidation induced by crude oil.


1- Нефтяная энциклопедия Казахстана, Алматы - 2005.

2- Справочник: Месторождении нефти и газа Казахстана, Алматы - 2007.

3- Белов ПС. Экология производства химических продуктов из углеводородов нефти и газа.- М., 1991.

4- Miklosovicova L, Trzilova B. Biodegradation of crude oil hydrocarbons in water environment. Biologia. (Bratislava), 46: 219 – 228. 1991.

5- Albers PH. Petroleum and individual polycyclic aromatic hydrocarbons. In, Handbook of Ecotoxicology. Hoffman, D.J., Rattner, B.A., Burton, G.A., and J. Cairns. (eds.) Chapter 15. Lewis Publishers, Boca Raton, Florida, pp 330 - 355. 1995.

6- National Research Council. Oil in the Sea: Inputs, Fates, and Effects. National Academy Press, Washington, DC, USA, pp. 7–10. 1985.

7- IARC. Monographs. Occupational Exposures in Petroleum Refining; Crude Oil and Major Petroleum Fuels, 45: 120. 1989.

8- Mansuy D. The great diversity of reactions catalyzed by cytochromes P450. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol, 121: 5-14. 1998.

9- Poland A, Knutson JC. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Ann Rev Pharmacol Toxicol, 22: 517-554. 1982.

10- Schenkman JB, Cinti DL. Preparation of microsomes with calcium. Methods Enzymol. 52: 83–89. 1978.

11- Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem. 239: 2370-2378. 1964.

12- Guengerich FP. Analysis and characterization of enzymes. In: Principles and methods of toxicology, Hayes A.W (eds). 3rd Edn., Raven Press, New York, pp 1259—1313. 1994.

13- Burlakova EB, Аlesenko AV, Molochkina EM, Palmina NP and Khrapova NG. Bioantioxidants for radiation damage and malignant growth. 214 pp. 1975.

14- Landu S, Everitt BS. A handbook of statistical Analyses using SPSS. Chapman and Hall/CRC press LLC. London, P.337. 2004.

15- Dipple A, Michejda CJ and Weisburger EK. Metabolism of chemical carcinogens. Pharmacol Ther, 27: 265-296. 1985.

16- Guengerich FP, Liebler DC. Enzymatic activation of chemicals to toxic metabolites. Crit Rev Toxicol, 14: 259–307. 1985.

17- Lu AY, West SB. Multiplicity of mammalian microsomal cytochromes P-450. Pharmacol Rev, 31: 277-295. 1980.

18- Wrighton SA, Stevens JC (1992). The human hepatic cytochromes P450 involved in drug metabolism. Crit Rev Toxicol, 22: 1-21. 1992.

19- Pyykko K., Paavilainen S, Metsa-Ketela T, Laustiola K. The increasing and decreasing effects of aromatic hydrocarbon solvents on pulmonary and hepatic cytochrome P-450 in the rat. Pharmacol Toxicol, 60: 288-293. 1987.

20- Toftgard R, Halpert J, Gustafsson JA. Xylene induces a cytochrome P-450 isozyme in rat liver similar to the major isozyme induced by phenobarbital. Mol Pharmacol, 23: 265-271. 1983.

21- Khan AA, Coppock RW, Schuler MM. Effects of multiple exposures of small doses of Pembina Cardium crude oil and diesel in rats. Arch Environ Contam Toxicol, 40: 418-424. 2001.

22- Downs CA, Shigenaka G, Fauth JE, Robinson CE, Huang A. Cellular physiological assessment of bivalves after chronic exposure to spilled Exxon Valdez crude oil using a novel molecular diagnostic biotechnology. Environ Sci Technol, 36: 2987-2993. 2002.

23- Liu J, Mori A. Involvement of reactive oxygen species in emotional stress: a hypothesis based on the immobilizations stress-induced oxidative damage and antioxidant defence changes in rat brain and the effect of antioxidant treatment with reduced glutathione. Int J Stress Mgt, 1: 249-263. 1994.

24- Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J, 1: 441-446. 1987.

25- Sies H. Oxidative stress: Introduction. In: Oxidative Stress:
oxidants and Antioxidants. H. Sies Ed, New York, NY, Academic Press. 1991.

26- Weyers AI, Ugnia LI, García Ovando H, Gorla NB. Ciprofloxacin increases hepatic and renal lipid hydroperoxides levels in mice. Bio cell, 26: 225-228. 2002.

27- Weyers A, Ugnia LI, Ovando HG, Gorla NB. Antioxidant capacity of vitamin C in mouse liver and kidney tissues. Bio cell, 32: 27-31. 2008.
How to Cite
MAHMOUD, K. E.; SHALAHMETOVA, T. M.; UMBAYEV, B. A.. Biochemical assessment of the effects of crude oil and ciprofloxacin intoxication on liver of male rats. International Journal of Biology and Chemistry, [S.l.], n. 3, p. 21-26, june 2012. ISSN 2409-370X. Available at: <>. Date accessed: 24 feb. 2019.


Crude oil; Ciprofloxacin; Cytochrome P-450 1A; Lipid peroxidation; Rat