RAPD analysis of genetic diversity in Berberis iliensis a species endemic to Kazakhstan
Keywords:
DNA (RAPD) markers, polymorphic bands, Shannon’s indexAbstract
Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity of Berberis iliensis (Berberidaceae), a species endemic to Kazakhstan and an important medicinal plant, collected from three populations along the Balkhash River. A total of 104 amplified bands were scored from the 4 RAPD primers, and a mean of 26 amplified bands per primer and 96% (100 bands) percentages of polymorphic bands (PPB) were found. The Shannon’s index was used to partition genetic diversity. Genetic diversity estimates indicated that 26 % of total diversity was among populations and 74 % within populations. In this study, the RAPD results of genetic diversity are similar to those of previous studies employing allozymes (17 % among populations and 83 % within populations). The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to more easily adapt to environmental variations.References
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Mukhitdinov, А.А. Ametov, A.K. Bissenbaev Analysis of genetic polymorphism of endemic Berberis iliensis population in Ile-Balkhash region of Kazakhstan
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3. Baitenov MS. In the world of rare plants. Alma-Ata. 1985. – 176 p.
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5. Karimov A, Shakirov R. Chemistry of Natural Compounds. 29(1): 69-70, 1993.
6. Nei M. Molecular evolutionary genetics. Columbia Univ. Press. 1987. – 512 p.
7. Atienzar FA, Jha AN. The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutation Research. 613: 76-102, 2006.
8. Lee SW, Ledig FT, Johnson DR. Genetic variation at allozyme and RAPD markers in Pinus longaeva (Pinaceae) of the White Mountains, California. Am. J. Bot. 89: 566-577, 2002.
9. Jover MA, del Castillo-Agudo L, Garcia-Carrascosa M, Segura J. Random amplified polymorphic DNA assessment of diversity in western Mediterranean populations of the seagrass Posidonia oceanica. Am. J. Bot. 90: 364–369, 2003.
10. Ester S, Sergio GN, Maurici M, Segura J. Population genetic study in the Balearic endemic plant species Digitalis minor (Scrophulaceae) using RAPD markers. Am. J. Bot. 88: 1750-1759, 2001.
11. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res.18: 6531–6535, 1990.
12. Nei M. Analysis of gene diversity in subdivided populations. Proc. Natl Acad. Sci. U.S.A. 70: 3321-3323, 1973.
13. Lewinton RC. The apportionment of human diversity. Evol. Biol. 6: 381-398, 1972.
14. Miller M.P. Tools for population genetic analyses (TFPGA) 1.3: A Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. 1997..
15. Hamrick JL, Godt HJW. Conservation genetics of endemic plant species. In Avise JC, Hamrick JL. Conservation Genetics: Case Studies from Nature. Chapman and Hall. 1996. - 281-304 p.
16. Fu CX, Qiu YX, Kong HH. RAPD analysis for genetic diversity in Changium smyrnioides (Apiaceae), an endangered plant. Bot. Bull. Acad. Sin. 44: 13–18, 2003.
17. B. Dzholdybaeva, N.A. Altybaeva, Aimbetov R.S., Smailov B., N.M.
Mukhitdinov, А.А. Ametov, A.K. Bissenbaev Analysis of genetic polymorphism of endemic Berberis iliensis population in Ile-Balkhash region of Kazakhstan
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Published
2012-06-20
How to Cite
Akishev, Z. D., I. T. Smekenov, A. N. Usenova, A. B. Duisenova, B. B. Smailov, N. M. Mukhidinov, and A. K. Bissenbaev. 2012. “RAPD Analysis of Genetic Diversity in Berberis Iliensis a Species Endemic to Kazakhstan”. International Journal of Biology and Chemistry, no. 3 (June):48-53. https://ijbch.kaznu.kz/index.php/kaznu/article/view/52.
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