Molecular study of Berberis iliensis M. Pop. and Berberis sphaerocarpa Kar. et Kir. wild populations in South-East Kazakhstan using ISSR markers

Abstract

B. iliensis is a rare endangered plant species with decreasing natural area. Interspecific hybrids B.iliensis × B.sphaerocarpa are even more rare phenomenon. In this work 6 populations of B.iliensis, 4 populations of B. sphaerocarpa and a single B. oblonga population are analysed. DNA from barberry leaves was extracted using SDS method. ISSR analysis was conducted with 5 primers. UPGMA tree and STRUCTURE diagram of 11 populations were constructed. Obtained results show relations between B.iliensis and B. sphaerocarpa in pure and hybrid populations and lay a first basis for further investigation of Kazakhstan barberry species.