Identification of flavonoids in plant samples by gas chromatography-mass spectrometry with pre-derivatization
DOI:
https://doi.org/10.26577/2218-7979-2016-9-1-46-51Keywords:
analysis, Artemisia, derivatization, flavonoids, gas chromatography, kaempferol, mass spectrometry, quercetinAbstract
Plants of Artemisia L. genus are important source of flavonoids – a group of biologically active substances, which are widely used in the perfume, alcoholic beverage, pharmaceutical industry and medicine. Flavonoids have antiseptic, anti-tumor, anti-inflammatory, antibacterial, antituberculosis actions. Therefore, testing and introduction of many types of Artemisia to the culture, and their selection in Kazakhstan is up-and-coming. Qualitative composition of wormwood Artemisia scopariaWaldst. et Kit flavonoids collected in Almaty and Ile-Balkhash region in 2013 year was studied by gas chromatography-mass spectrometry method. Samples were prepared by extraction three times with 40% ethanol solution. During the identification of flavonoids by gas chromatography-mass spectrometry pre-derivatization of the compounds was carried out by N,O-Bis(trimethylsilyl) trifluoroacetamide, which is the most reactive derivatizing agent to increase the volatility and thermal stabilityofstudied substances. Kaempferol and quercetin were found as a result of analysis.References
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3. ZhengJ., YangB., TuomasjukkaS., OuSh., KallioH.(2009) J. Agric. Food Chem57:2977-2987.
4. Creaser C., Koupai-Abyazani M., Stephenson G.(1989) J. Chromatogrol. 478:415-421.
5. Fenner G. (1996) J. Agric. Food Chem. 44:3727-3729.
6. ZhangK., Zuo Y.(2004) J. Agric. Food Chem.52:222-227.
7. AnliErtan, VuralN., Kizilet E.(2008) JI Brewing. 114:239-245
8. Garcia-VillalbaR., PacchiarottaT., Carrasco-PancorboA., Segura-CarreteroA., Fernandez-GutierrezA., DeciderA., MayborodaO.(2011) J.Chromatogr.1218:959-971.
9. Loots D., van der WesthuizenF., BotesL.(2007) J. Agric. Food Chem. 55:6891-6896.
10. Soleas G., Diamandis E., Karumanchiri A., Goldberg D.(1997) Anal. Chem. 69:4405-4409.
11. Horvat R., Senter S.(1980) J. Agric. Foal Chem. 28:1292-1295.
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13. Kashutin M., Ioffe S., Tartakovsky V.(1975) Usp9:1620-1648.
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How to Cite
Alimzhanova, M., and D. Makhayeva. 2016. “Identification of Flavonoids in Plant Samples by Gas Chromatography-Mass Spectrometry With Pre-Derivatization”. International Journal of Biology and Chemistry 9 (1):46-51. https://doi.org/10.26577/2218-7979-2016-9-1-46-51.
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